Microtransplantation of acetylcholine receptors from normal or denervated rat skeletal muscles to frog oocytes
Por:
Bernareggi A., Reyes-Ruiz J.M., Lorenzon P., Ruzzier F., Miledi R.
Publicada:
1 mar 2011
Resumen:
Non-technical summary Nicotinic acetylcholine receptors (nAChRs) expressed by skeletal muscle are the key proteins of the motoneuron-muscle communication necessary to induce muscle contraction. The biophysical and pharmacological characterization of the receptors in adult human skeletal muscle is limited by the difficulties associated with obtaining, and maintaining, suitable biopsy material. In this paper, we characterized some nAChR properties of innervated and denervated skeletal muscle by injecting the membranes into Xenopus oocytes. Such an approach, termed the microtransplantation technique, offers two main advantages: (1) direct characterization of the original receptors, still embedded in their natural lipid environment with their associated molecules; and (2) the possibility of using membranes isolated from postmortem frozen tissues. We demonstrate that this technique can be a very simple and useful approach to study skeletal muscle receptors and ion channels under different p
Filiaciones:
Bernareggi A.:
Department of Life Sciences and Centre for Neuroscience B.R.A.I.N., University of Trieste, via Fleming 22, I-34127 Trieste, Italy
Reyes-Ruiz J.M.:
Department of Neurobiology and Behaviour, University of California, Irvine, CA 92697-4550, United States
Lorenzon P.:
Department of Life Sciences and Centre for Neuroscience B.R.A.I.N., University of Trieste, via Fleming 22, I-34127 Trieste, Italy
Ruzzier F.:
Department of Life Sciences and Centre for Neuroscience B.R.A.I.N., University of Trieste, via Fleming 22, I-34127 Trieste, Italy
Miledi R.:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Queretaro, Mexico
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