Trypanosoma cruzi: Multiple actin isovariants are observed along different developmental stages
Por:
Cevallos A.M., Segura-Kato Y.X., Merchant-Larios H., Manning-Cela R., Hernandez-Osorio, LA, Márquez-Dueñas C., Ambrosio J.R., Reynoso-Ducoing O., Hernández R.
Publicada:
1 ene 2011
Resumen:
The expression and biological role of actin during the Trypanosoma cruzi life cycle remains largely unknown. Polyclonal antibodies against a recombinant T. cruzi actin protein were used to confirm its expression in epimastigotes, trypomastigotes, and amastigotes. Although the overall levels of expression were similar, clear differences in the subcellular distribution of actin among the developmental stages were identified. The existence of five actin variants in each developmental stage with distinct patterns of expression were uncovered by immunoblotting of protein extracts separated 2D-SDS gels. The isoelectric points of the actin variants in epimastigotes ranged from 4.45 to 4.9, whereas they ranged from 4.9 to 5.24 in trypomastigotes and amastigotes. To determine if the actin variants found could represent previously unidentified actins, we performed a genomic survey of the T.cruzi GeneDB database and found 12 independent loci encoding for a diverse group of actins and actin-like proteins that are conserved among trypanosomatids. © 2010 Elsevier Inc.
Filiaciones:
Cevallos A.M.:
Univ Nacl Autonoma Mexico, Dept Biol Mol & Biotecnol, Mexico City 04510, DF, Mexico
Segura-Kato Y.X.:
Univ Nacl Autonoma Mexico, Dept Biol Mol & Biotecnol, Mexico City 04510, DF, Mexico
Merchant-Larios H.:
Univ Nacl Autonoma Mexico, Dept Biol Celular & Fisiol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Manning-Cela R.:
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 14-740, 07000 México, DF, Mexico
Márquez-Dueñas C.:
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 14-740, 07000 México, DF, Mexico
Ambrosio J.R.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Reynoso-Ducoing O.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, DF, Mexico
Hernández R.:
Univ Nacl Autonoma Mexico, Dept Biol Mol & Biotecnol, Mexico City 04510, DF, Mexico
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