Ribotype and serotype diversity of Escherichia coli isolates from poultry and human sources
Por:
Sheffield C., Rosario-Cortés C., Bischoff K.
Publicada:
1 jul 2010
Resumen:
Escherichia coli isolates from the Universidad Nacional Autonoma de Mexico were analyzed to evaluate the genetic relatedness and serotype. Ribotype was determined using the RiboPrinter (R) Microbial Characterization System following DNA digestion with the restriction enzyme EcoRI. A total of 41 ribogroups were isolated. These segregated into three clusters, one cluster contained only poultry isolates, the second contained only human isolates and the third contained 55% poultry isolates and 45% human isolates. Results showed a high level of ribotypic relatedness within the E. coli isolates that could infect both poultry and human sources. E. coli isolates collected from exclusively poultry or human sources showed a high level of ribotypic diversity. Serotype was determined using antisera against somatic (0) antigens. A total of 22 serotypes were identified, of which six were typical of the avian pathogenic E. coli (APEC) strains and six contained both human and poultry isolates.
Filiaciones:
Sheffield C.:
USDA, ARS, Southern Plains Agriculture Research Center, 2881 FandBRoad, College Station, TX 77845, United States
Rosario-Cortés C.:
Univ Nacl Autonoma Mexico, Dept Anim Prod, Fac Med Vet & Zootecnia, Mexico City 04510, DF, Mexico
Bischoff K.:
National Center for Agricultural Utilization Research, USDA Agricultural Research Service, Peoria, IL 61603, United States
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