Gene Regulation by BCL3 in a Cervical Cancer Cell Line
Por:
Maldonado V., Espinosa M., Pruefer F., Patino, N, Ceballos-Cancino G., Urzua U., Juretic N., Melendez-Zajgla J.
Publicada:
1 ene 2010
Resumen:
BCL3 is a putative proto-oncogene deregulated in haematopoieitic and solid tumours. It has been suggested that its oncogenic effects could be mediated, at least in part, by inducing proliferation and inhibiting cell death. To provide more insight into the mediators of these effects, we used an unbiased approach to analyse the mRNA expression changes after knocking-down BCL3 using specific shRNAs. One hundred eighty genes were up-regulated and sixtynine genes were down-regulated after knocking down BCL3. Function analyses showed enrichment in genes associated with cellular growth and proliferation, cell death and gene expression. We found that STAT3, an important oncogene in human cancer, was the central node of one of the most significant networks. We validated STAT3 as a bona fide target of BCL3 by additional interference RNA and in silico analyses of previously reported lymphoma patients.
Filiaciones:
Maldonado V.:
Molecular Biology Laboratory, Instituto Nacional de Cancerologia, Av. San Fernando 22, Tlalpan 14080, Mexico City, Mexico
National Institute of Genomic Medicine, Periferico Sur No. 4124, Torre Zafiro II, Col. Ex Rancho de Anzaldo, Alvaro Obregón, 01900, Mexico, Mexico
Espinosa M.:
Cancer Functional Genomics Laboratory, National Institute of Genomic Medicine, Mexico City, Mexico
Pruefer F.:
Cancer Functional Genomics Laboratory, National Institute of Genomic Medicine, Mexico City, Mexico
Ceballos-Cancino G.:
Cancer Functional Genomics Laboratory, National Institute of Genomic Medicine, Mexico City, Mexico
Urzua U.:
PBCM, ICBM Facultad de Medicina, Universidad de Chile, Santiago, Chile
Juretic N.:
PBCM, ICBM Facultad de Medicina, Universidad de Chile, Santiago, Chile
Melendez-Zajgla J.:
Cancer Functional Genomics Laboratory, National Institute of Genomic Medicine, Mexico City, Mexico
National Institute of Genomic Medicine, Periferico Sur No. 4124, Torre Zafiro II, Col. Ex Rancho de Anzaldo, Alvaro Obregón, 01900, Mexico, Mexico
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