Bcl-2 overexpression in hepatic stellate cell line CFSC-2G, induces a pro-fibrotic state
Por:
Gonzalez-Puertos, VY, Hernandez-Perez, E, Nuno-Lambarri, N, Ventura-Gallegos, JL, Lopez-Diazguerrero, NE, Robles-Diaz, G, Gutierrez-Ruiz, MC, Konigsberg, M
Publicada:
1 jul 2010
Resumen:
Background and Aim: Development of hepatic fibrosis is a complex process that involves oxidative stress (OS) and an altered balance between pro- and anti-apoptotic molecules. Since Bcl-2 overexpression preserves viability against OS, our objective was to address the effect of Bcl-2 overexpression in the hepatic stellate cells (HSC) cell-line CFSC-2G under acetaldehyde and H(2)O(2) challenge, and explore if it protects these cells against OS, induces replicative senescence and/or modify extracellular matrix (ECM) remodeling potential. Methods: To induce Bcl-2 overexpression, HSC cell line CFSC-2G was transfected by lipofection technique. Green fluorescent protein-only CFSC-2G cells were used as a control. Cell survival after H(2)O(2) treatment and total protein oxidation were assessed. To determine cell cycle arrest, proliferation-rate, DNA synthesis and senescence were assessed. Matrix metalloproteinases (MMP), tissue-inhibitor of MMP (TIMP), transglutaminases (TG) and smooth muscle a-
Filiaciones:
Ventura-Gallegos, JL:
Independent Natl Univ Mexico, Genom Med Dept, Mexico City, DF, Mexico
Independent Natl Univ Mexico, Dept Biochem, Natl Inst Med Sci & Rescuing Nutr Salvador Zubira, Mexico City, DF, Mexico
Robles-Diaz, G:
Independent Natl Univ Mexico, Fac Med, Expt Med Unit, Mexico City, DF, Mexico
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