PATHWAYS INVOLVED IN THE GENERATION OF REACTIVE OXYGEN AND NITROGEN SPECIES DURING GLUCOSE DEPRIVATION AND ITS ROLE ON THE DEATH OF CULTURED HIPPOCAMPAL NEURONS
Por:
Paramo, B, Hernandez-Fonseca, K, Estrada-Sanchez, AM, Jimenez, N, Hernandez-Cruz, A, Massieu, L
Publicada:
2 jun 2010
Categoría:
Neuroscience (miscellaneous)
Resumen:
Oxidative stress has been suggested as a mechanism contributing to neuronal death induced by hypoglycemia, and an early production of reactive species (RS) during the hypoglycemic episode has been observed. However, the sources of reactive oxygen (ROS) and nitrogen (RNS) species have not been fully identified. In the present study we have examined the contribution of various enzymatic pathways to RS production and neuronal death induced by glucose deprivation (GD) in hippocampal cultures. We have observed a rapid increase in RS during GD, which depends on the activation of NMDA and non-NMDA receptors and on the influx of calcium from the extracellular space. Accordingly, intracellular calcium concentration [Ca(2+)](i) progressively increases more than 30-fold during the GD period. It was observed that superoxide production through the activation of the calcium-dependent enzymes, phospholipase A(2) (cPLA(2)) and xanthine oxidase (XaO), contributes to neuronal damage, while nitric oxide
Filiaciones:
Paramo, B:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neuropatol Mol, Div Neurociencias, Mexico City 04510, DF, Mexico
Hernandez-Fonseca, K:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neuropatol Mol, Div Neurociencias, Mexico City 04510, DF, Mexico
Estrada-Sanchez, AM:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neuropatol Mol, Div Neurociencias, Mexico City 04510, DF, Mexico
Jimenez, N:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurociencia Cognit, Mexico City 04510, DF, Mexico
Hernandez-Cruz, A:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neurociencia Cognit, Mexico City 04510, DF, Mexico
Massieu, L:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Neuropatol Mol, Div Neurociencias, Mexico City 04510, DF, Mexico
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