Synthesis of 26-hydroxy-22-oxocholestanic frameworks from diosgenin and hecogenin and their in vitro antiproliferative and apoptotic activity on human cervical cancer CaSki cell
Por:
Fernández-Herrera M.A., López-Muñoz H., Hernandez-Vazquez, JMV, López-Dávila M., Escobar-Sánchez M.L., Sánchez-Sánchez L., Pinto B.M., Sandoval-Ramírez J.
Publicada:
1 abr 2010
Resumen:
Certain steroidal compounds have demonstrated an antiproliferative effect against several tumor cell lines; however, their complete role on cancer cells is not currently established. Herein, we report the synthesis and evaluation of two new 26-hydroxy-22-oxocholestanic steroids on cervical cancer CaSki cells. The title compounds were prepared from diosgenin and hecogenin in excellent yields. We determined their effect on cell proliferation, cell cycle, and cell death. The cytotoxic effect of the title compounds on CaSki and human lymphocytes was also evaluated, indicating that the main cell death process is not necrosis; the null effect on lymphocytes implies that they are not cytotoxic. The observation of apoptotic bodies as well as the increase in the expression of active caspase-3 along with the fragmentation of DNA confirmed that such new cholestanic frameworks induced apoptosis in tumor cells. Significantly, their antiproliferative activity on tumor cells did not affect the prolif
Filiaciones:
Fernández-Herrera M.A.:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Ciudad Universitaria, 72570 Puebla, Pue., Mexico
López-Muñoz H.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Mexico City 09230, DF, Mexico
Hernandez-Vazquez, JMV:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Mexico City 09230, DF, Mexico
López-Dávila M.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Mexico City 09230, DF, Mexico
Escobar-Sánchez M.L.:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Mexico City 04510, DF, Mexico
Sánchez-Sánchez L.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Mexico City 09230, DF, Mexico
Pinto B.M.:
Department of Chemistry, Simon Fraser University, Burnaby, BC V5A 1S6, Canada
Sandoval-Ramírez J.:
Facultad de Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Ciudad Universitaria, 72570 Puebla, Pue., Mexico
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