Degraded Carrageenan Causing Colitis in Rats Induces TNF Secretion and ICAM-1 Upregulation in Monocytes through NF-kappa B Activation
Por:
Benard C., Cultrone A., Michel C., Rosales C., Segain, JP, Lahaye M., Galmiche, JP, Cherbut C., Blottiere, HM
Publicada:
13 ene 2010
Resumen:
Carrageenan (CGN) is a high molecular weight sulphated polysaccharide derived from red seaweeds. In rodents, its degraded forms (dCGN) can induce intestinal inflammation associated with macrophage recruitment and activation. The aim of this study was: 1) to analyze the size-dependent effects of dCGN on colon inflammation in vivo, and 2) to correlate these effects with monocyte/macrophage proliferation, cytokine production and expression of various cell surface antigens including ICAM-1 adhesion molecule. Peripheral blood monocytes (PBM) and THP-1 monocytic cells were cultured in the presence of either 10 or 40 kDa, dCGN. The 40 kDa, but not the 10 kDa dCGN, induced colitis in in vivo. Degraded CGN inhibited THP-1 cell proliferation in vitro, arresting the cells in G1 phase. In addition, dCGN increased ICAM-1 expression in both PBM and THP-1 cells with a major effect seen after 40 kDa dCGN exposure. Also, dCGN stimulated monocyte aggregation in vitro that was prevented by incubation wit
Filiaciones:
Benard C.:
INRA, UR910 UEPSD, Domaine de Vilvert, Jouy-en-Josas, France
Pharmanet, Paris, France
Cultrone A.:
INRA, UR910 UEPSD, Domaine de Vilvert, Jouy-en-Josas, France
Michel C.:
UMR 1280 PHAN, Université de Nantes, CHU Hôtel-Dieu, Nantes, France
Rosales C.:
Univ Nacl Autonoma Mexico, Dept Immunol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Lahaye M.:
INRA, BIA, Nantes, France
Cherbut C.:
UMR 1280 PHAN, Université de Nantes, CHU Hôtel-Dieu, Nantes, France
Nestle Research Centre, Lausanne, France
Gold
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