Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities
Por:
Orencio-Trejo, M, Flores, N, Escalante, A, Hernandez-Chavez, G, Bolivar, F, Gosset, G, Martinez, A
Publicada:
1 may 2008
Resumen:
Background: A metabolic regulation study was performed, based upon measurements of enzymatic activities, fermentation performance, and RT-PCR analysis of pathways related to central carbon metabolism, in an ethanologenic Escherichia coli strain (CCE14) derived from lineage C. In comparison with previous engineered strains, this E .coli derivative has a higher ethanol production rate in mineral medium, as a result of the elevated heterologous expression of the chromosomally integrated genes encoding PDC(Zm) and ADH(Zm) ( pyruvate decarboxylase and alcohol dehydrogenase from Zymomonas mobilis). It is suggested that this behavior might be due to lineage differences between E. coli W and C. Results: This study demonstrated that the glycolytic flux is controlled, in this case, by reactions outside glycolysis, i.e., the fermentative pathways. Changes in ethanol production rate in this ethanologenic strain result in low organic acid production rates, and high glycolytic and ethanologenic flux
Filiaciones:
Orencio-Trejo, M:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62191, Morelos, Mexico
Flores, N:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62191, Morelos, Mexico
Escalante, A:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62191, Morelos, Mexico
Hernandez-Chavez, G:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62191, Morelos, Mexico
Bolivar, F:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62191, Morelos, Mexico
Gosset, G:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62191, Morelos, Mexico
Martinez, A:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Ingn Celular & Biocatalisis, Cuernavaca 62191, Morelos, Mexico
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