Cyclosporin A Inhibits UV-Radiation-Induced Membrane Damage but is Unable to Inhibit Carboxyatractyloside-Induced Permeability Transition
Por:
García N., Zazueta C., El-Hafidi M., Pavón N., Martínez-Abundis E., Hernández-Esquivel L., Chávez E.
Publicada:
1 nov 2009
Resumen:
This work was undertaken to gain further information on the chemical characteristics of the membrane entity involved in the formation of the nonspecific pore. Mitochondria were subjected to oxidative stress by exposure to UV radiation. The results indicate that ultraviolet C radiation induces structural modifications in the adenine nucleotide translocase that lead to membrane permeability transition. Membrane leakage was assessed by measuring mitochondrial Ca2+ transport, the transmembrane electric gradient, and mitochondrial swelling. UV-irradiated mitochondria were unable to retain matrix Ca2+ or to maintain a high level of membrane potential when Ca2+ was added; furthermore, UV-irradiated mitochondria underwent large amplitude swelling. Release of cytochrome c and formation of malondialdehyde, owing to lipid peroxidation, were also seen. Structural modifications of the translocase were revealed by an increase in the binding of the fluorescent probe eosin-5-maleimide to thiol residue
Filiaciones:
García N.:
Departamento de Bioquímica, Instituto Nacional de Cardiologia, Juan Badiano # 1, Ignacio Chávez, Tlalpam, D.F. 014080, Mexico
Zazueta C.:
Departamento de Bioquímica, Instituto Nacional de Cardiologia, Juan Badiano # 1, Ignacio Chávez, Tlalpam, D.F. 014080, Mexico
El-Hafidi M.:
Departamento de Bioquímica, Instituto Nacional de Cardiologia, Juan Badiano # 1, Ignacio Chávez, Tlalpam, D.F. 014080, Mexico
Pavón N.:
Departamento de Bioquímica, Instituto Nacional de Cardiologia, Juan Badiano # 1, Ignacio Chávez, Tlalpam, D.F. 014080, Mexico
Martínez-Abundis E.:
Departamento de Bioquímica, Instituto Nacional de Cardiologia, Juan Badiano # 1, Ignacio Chávez, Tlalpam, D.F. 014080, Mexico
Hernández-Esquivel L.:
Departamento de Bioquímica, Instituto Nacional de Cardiologia, Juan Badiano # 1, Ignacio Chávez, Tlalpam, D.F. 014080, Mexico
Chávez E.:
Departamento de Bioquímica, Instituto Nacional de Cardiologia, Juan Badiano # 1, Ignacio Chávez, Tlalpam, D.F. 014080, Mexico
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