Comparative Proteomic Analysis of Helicobacter pylori-Expressed Proteins in Gastric Epithelial Cell Apoptosis
Por:
Gonzalo, CR, Adriana, VB, Guillermo, MH, Xochitl V.M., Ruben, AGG, Yolanda, LV
Publicada:
1 oct 2009
Resumen:
Helicobacter pylori infection induces an increase in the rate of apoptosis in gastric epithelial cells and initiates a secondary hyperproliferative response. Currently, cytotoxin-associated proteins and vacuolating cytotoxin participate in the induction of the apoptotic process in the gastric epithelium, however, the existence of other factors that participate in this process has not been ruled out. Moreover, differential protein expression by H. pylori during the apoptotic process has not been studied. The aim of this work was to study differential H. pylori protein expression during the process of apoptosis in AGS cell. AGS cells were infected with H. pylori and apoptosis was determined by measuring chromatin condensation and caspase-3 activity. In addition, bacterial and cellular protein extraction was performed during apoptosis. The H. pylori protein profile was analyzed by double-dimension electrophoresis (2-DE) and protein identification was performed by LC/NSI-MS/MS analysis. A
Filiaciones:
Gonzalo, CR:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Programa Inmunol Mol Microbiana, Mexico City 04510, DF, Mexico
Adriana, VB:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Programa Inmunol Mol Microbiana, Mexico City 04510, DF, Mexico
Guillermo, MH:
Univ Nacl Autonoma Mexico, Fac Med, Lab Peptidos & Prot, Dept Bioquim, Mexico City 04510, DF, Mexico
Xochitl V.M.:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Programa Inmunol Mol Microbiana, Mexico City 04510, DF, Mexico
Yolanda, LV:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Programa Inmunol Mol Microbiana, Mexico City 04510, DF, Mexico
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