SEGREGATION OF MET-ENKEPHALIN FROM VESICULAR ACETYLCHOLINE TRANSPORTER AND CHOLINE ACETYLTRANSFERASE IN SYMPATHETIC PREGANGLIONIC VARICOSITIES MOSTLY LACKING SYNAPTOPHYSIN AND S
Por:
Samano, C, Zetina, ME, Cifuentes, F, Morales, MA
Publicada:
29 sep 2009
Categoría:
Neuroscience (miscellaneous)
Resumen:
Sympathetic preganglionic neurons (SPN) coexpress the acetylcholine (ACh)-synthesizing enzyme choline acetyltransferase and different peptides in their cell bodies, but can express them independently in separate varicosities, indicating that SPN segregate transmitters to different synapses. Consequently, there are populations of preganglionic varicosities (peptidergic and noncholinergic) that store peptides but not ACh. We studied in the cell bodies and axon processes of the rat SPN the expression and the proportional coexpression of the vesicular ACh transporter-like immunoreactivity (VAChT), a specific marker of cholinergic synaptic vesicles or ChAT-like immunoreactivity (ChAT), and the peptide methionine enkephalin-like immunoreactivity (mENK), and confirmed the presence of a population of SPN peptidergic, noncholinergic varicosities. We characterized these varicosities by exploring the occurrence of synaptophysin-like immunoreactivity (Syn), a marker of small clear vesicles, and sy
Filiaciones:
Samano, C:
Univ Nacl Autonoma Mexico, Dept Biol Celular & Fisiol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Zetina, ME:
Univ Nacl Autonoma Mexico, Dept Biol Celular & Fisiol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Cifuentes, F:
Univ Nacl Autonoma Mexico, Dept Biol Celular & Fisiol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Morales, MA:
Univ Nacl Autonoma Mexico, Dept Biol Celular & Fisiol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
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