Induction of focal epithelial hyperplasia in tongue of young bk6-E6/E7 HPV16 transgenic mice
Por:
Ocadiz-Delgado R., Marroquin-Chavira A., Hernandez-Mote R., Valencia C., Manjarrez-Zavala M.E., Covarrubias L., Gariglio P.
Publicada:
1 ago 2009
Resumen:
Squamous cell carcinoma (SCC) of the oral cavity is one of the most common neoplasms in the world. During the past 2 decades, the role of high-risk human papilloma virus (HR-HPV) has been studied and the data supporting HPV as a one of the causative agents in the development and progression of a sub-set of head and neck squamous cell carcinomas (HNSCC) has accumulated. In order to investigate the role of HR-HPV oncogene expression in early epithelial alterations in vivo, we produced transgenic mice expressing HPV16 early region genes from the promoter of the bovine keratin 6 gene (Tg[bK6-E6/E7]). In this article, we demonstrate that E6/E7 transgene was abundantly expressed and cellular proliferation was increased in the middle tongue epithelia of transgenic mice, and that in the same region young (27 weeks old) Tg[bK6-E6/E7] mice spontaneously developed histological alterations, mainly focal epithelial hyperplasia (FEH). © Springer Science+Business Media B.V. 2009.
Filiaciones:
Ocadiz-Delgado R.:
Department of Genetics and Molecular Biology, CINVESTAV-IPN Mexico, Av. IPN 2508. Colonia San Pedro Zacatenco, CP 07360 Mexico, DF, Mexico
Marroquin-Chavira A.:
Department of Genetics and Molecular Biology, CINVESTAV-IPN Mexico, Av. IPN 2508. Colonia San Pedro Zacatenco, CP 07360 Mexico, DF, Mexico
Hernandez-Mote R.:
Department of Pathology, Hospital Infantil de Mexico Federico Gómez, Mexico, Dr. Marquez 162. Col. Doctores, CP 06720 Mexico, DF, Mexico
Valencia C.:
Univ Nacl Autonoma Mexico, Inst Biotechnol, Cuernavaca 62210, Morelos, Mexico
Manjarrez-Zavala M.E.:
Instituto Nacional de Enfermedades Respiratorias (INER), Mexico, Calzada de Tlalpan 4502. Col. Sección XVI, CP 14080 Mexico, DF, Mexico
Covarrubias L.:
Univ Nacl Autonoma Mexico, Inst Biotechnol, Cuernavaca 62210, Morelos, Mexico
Gariglio P.:
Department of Genetics and Molecular Biology, CINVESTAV-IPN Mexico, Av. IPN 2508. Colonia San Pedro Zacatenco, CP 07360 Mexico, DF, Mexico
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