Phenotypic differences between BCG vaccines at the proteome level
Por:
Rodríguez-Alvarez M., Mendoza-Hernández G., Encarnación S., Calva J.J., López-Vidal Y.
Publicada:
1 mar 2009
Resumen:
To contribute to Mycobacterium bovis BCG characterization, two substrains were analyzed using two-dimensional gel electrophoresis (2D-PAGE) and mass spectrometry (MS), based on their protective efficacy in a pulmonary-tuberculosis mouse model. Cell-fraction proteins of BCG Denmark and Phipps substrains were separated into - 500 spots in 2D-PAGE. The proteomes were similar in protein number, and isoelectric point (pl) and molecular mass (MM) distribution. Statistical analysis, resulted in 72 spots with no change, and 168 and 90 unique for BCG Phipps or Denmark, respectively. Two hundred and fourteen spots showed changes in intensity of >1-fold, 138 of Denmark, and 76 of Phipps. Seventeen spots were selected for MS-based identification (13 from Phipps and 4 from Denmark), including unique, as well as proteins with changes in intensity. The proteins identified participate in virulence, detoxification, adaptation, lipid metabolism, information pathways, cell wall and cell processes, interm
Filiaciones:
Rodríguez-Alvarez M.:
Univ Nacl Autonoma Mexico, Programa Inmunol Mol Microbiana, Dept Microbiol & Parasitol, Fac Med, Mexico City 04510, DF, Mexico
Mendoza-Hernández G.:
Univ Nacl Autonoma Mexico, Lab Peptidos & Prot, Dept Bioquim, Fac Med, Mexico City 04510, DF, Mexico
Encarnación S.:
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Lab Ingn Metab, Cuernavaca 62210, Morelos, Mexico
Calva J.J.:
Unidad de Epidemiología Clínica, Instituto Nacional de Ciencias Medicas y de la Nutrición Salvador Zubirán (INCMNSZ), Vasco de Quiroga #15, Colonia Seccion XVI, Tlalpan, 14000 Mexico, D.F., Mexico
López-Vidal Y.:
Univ Nacl Autonoma Mexico, Programa Inmunol Mol Microbiana, Dept Microbiol & Parasitol, Fac Med, Mexico City 04510, DF, Mexico
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