SnRK1 Isoforms AKIN10 and AKIN11 Are Differentially Regulated in Arabidopsis Plants under Phosphate Starvation
Por:
Fragoso, S, Espindola, L, Paez-Valencia, J, Gamboa, A, Camacho, Y, Martinez-Barajas, E, Coello, P
Publicada:
1 abr 2009
Resumen:
During phosphate starvation, Snf1-related kinase 1 (SnRK1) activity significantly decreases compared with plants growing under normal nutritional conditions. An analysis of the expression of the genes encoding for the catalytic subunits of SnRK1 showed that these subunits were not affected by phosphate starvation. Transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing the AKIN10 and AKIN11 catalytic subunits fused with green fluorescent protein (GFP) were produced, and their localizations were mainly chloroplastic with low but detectable signals in the cytoplasm. These data were corroborated with an immunocytochemistry analysis using leaf and root sections with an anti-AKIN10/AKIN11 antibody. The SnRK1 activity in transgenic plants overexpressing AKIN11-GFP was reduced by 35% to 40% in phosphate starvation, in contrast with the results observed in plants overexpressing AKIN10-GFP, which increased the activity by 100%. No differences in activity were observed in plants grow
Filiaciones:
Fragoso, S:
Univ Nacl Autonoma Mexico, Dept Bioquim, Fac Quim, Mexico City 04510, DF, Mexico
Espindola, L:
Univ Nacl Autonoma Mexico, Dept Bioquim, Fac Quim, Mexico City 04510, DF, Mexico
Paez-Valencia, J:
Univ Nacl Autonoma Mexico, Dept Ecol Func, Inst Ecol, Mexico City 04510, DF, Mexico
Gamboa, A:
Univ Nacl Autonoma Mexico, Dept Ecol Func, Inst Ecol, Mexico City 04510, DF, Mexico
Camacho, Y:
Univ Nacl Autonoma Mexico, Dept Bioquim, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Martinez-Barajas, E:
Univ Nacl Autonoma Mexico, Dept Bioquim, Fac Quim, Mexico City 04510, DF, Mexico
Coello, P:
Univ Nacl Autonoma Mexico, Dept Bioquim, Fac Quim, Mexico City 04510, DF, Mexico
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