The lateral root initiation index: an integrative measure of primordium formation
Por:
Dubrovsky J.G., Soukup A., Napsucialy-Mendivil S., Jeknic Z., Ivanchenko M.G.
Publicada:
1 mar 2009
Categoría:
Plant Science
Resumen:
Lateral root initiation is an essential and continuous process in the formation of root systems; therefore, its quantitative analysis is indispensable. In this study a new measure of lateral root initiation is proposed and analysed, namely the lateral root initiation index (I(LRI)), which defines how many lateral roots and/or primordia are formed along a parent-root portion corresponding to 100 cortical cells in a file. For data collection, a commonly used root clearing procedure was employed, and a new simple root clearing procedure is also proposed. The I(LRI) was determined as 100dl, where d is the density of lateral root initiation events (number mm(-1)) and l is the average fully elongated cortical cell length (mm). Analyses of different Arabidopsis thaliana genotypes and of a crop plant, tomato (Solanum lycopersicum), showed that I(LRI) is a more precise parameter than others commonly used as it normalizes root growth for variations in cell length. Lateral root primordium density
Filiaciones:
Dubrovsky J.G.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62250, Morelos, Mexico
Soukup A.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62250, Morelos, Mexico
Napsucialy-Mendivil S.:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62250, Morelos, Mexico
Jeknic Z.:
Department of Horticulture, ALS 4017, Oregon State University, Corvallis, OR 97331, United States
Ivanchenko M.G.:
Department of Botany and Plant Pathology, 2082 Cordley Hall, Oregon State University, Corvallis, OR 97331, United States
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