Characterization of langerhans cells in epidermal sheets along the body of Armadillo (Dasypus novemcinctus)
Por:
Quesada-Pascual F., Jimenez-Flores R., Flores-Langarica A., Silva-Sanchez A., Calderon-Amador J., Rene, MC, Limon-Flores A.Y., Estrada-Parra S., Santos-Argumedo L., Estrada-Garcia I., Flores-Romo L.
Publicada:
15 ago 2008
Resumen:
Armadillos are apparently important reservoirs of Mycobacterium leprae and an animal model for human leprosy, whose immune system has been poorly studied. We aimed at characterizing the armadillo's langerhans cells (LC) using epidermal sheets instead of tissue sections, since the latter restrict analysis only to cut-traversed cells. Epidermal sheets by providing an en face view, are particularly convenient to evaluate dendritic morphology (cells are complete), spatial distribution (regular vs. clustered), and frequency (cell number/tissue area). Lack of anti-armadillo antibodies was overcome Using LC-restricted ATPase staining, allowing assessment of cell frequency, cell size, and dendrites extension. Average LC frequency in four animals was 528 LC/mm(2), showing a rather uniform non-clustered distribution, which increased towards the animal's head, while cell size increased towards the tail: without overt differences between sexes. The screening of antibodies to human DC (MHC-II. CD1a
Filiaciones:
Quesada-Pascual F.:
Department of Immunology, National School, Biological Sciences ENCB-IPN, Mexico City, Mexico
Jimenez-Flores R.:
Univ Nacl Autonoma Mexico, Natl Univ Mexico, Fac High Studies Iztacala, Mexico City, DF, Mexico
Flores-Langarica A.:
Department of Immunology, National School, Biological Sciences ENCB-IPN, Mexico City, Mexico
Silva-Sanchez A.:
Department of Immunology, National School, Biological Sciences ENCB-IPN, Mexico City, Mexico
Calderon-Amador J.:
Department of Cell Biology, CINVESTAV-IPN, Mexico City, Mexico
Rene, MC:
Univ Nacl Autonoma Mexico, Natl Univ Mexico, Fac High Studies Iztacala, Mexico City, DF, Mexico
Limon-Flores A.Y.:
Department of Cell Biology, CINVESTAV-IPN, Mexico City, Mexico
Estrada-Parra S.:
Department of Immunology, National School, Biological Sciences ENCB-IPN, Mexico City, Mexico
Santos-Argumedo L.:
Department of Molecular Biomedicine, CINVESTAV-IPN, México City, Mexico
Estrada-Garcia I.:
Department of Immunology, National School, Biological Sciences ENCB-IPN, Mexico City, Mexico
Flores-Romo L.:
Department of Cell Biology, CINVESTAV-IPN, Mexico City, Mexico
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