Association of interleukin-6 gene Polymorphisms with bone mineral density in Mexican women
Por:
Magana, JJ, Gómez R., Cisneros B., Casas L., Valdés-Flores M.
Publicada:
1 ago 2008
Categoría:
Medicine (miscellaneous)
Resumen:
Background. The interleukin-6 gene (IL-6) stimulates osteoclast development; therefore, it has been implicated in osteoporosis. In this study, the association of osteoporosis with three IL-6 gene markers (a CA dinucleotide repeat and two single nucleotide polymorphisms [SNPs]: G-174C and G-572C) was tested in the Mexican population. Methods. Population sample was comprised of 70 osteoporotic women, 70 non-osteoporotic women, and 500 subjects from the general population who were genotyped for the IL-6 markers. SNPs were analyzed by real-time PCR using the 5' exonuclease assay, whereas the CA dinucleotide polymorphism was evaluated by PCR and capillary electrophoresis. The allele -phenotype relationship was analyzed with the statistical method STRAT that considered population stratification and the results were adjusted with potential confounders for osteoporosis by a longitudinal multivariate model. Results. We found that the C allele of the G-174C SNP and the A3 allele of the CA polymo
Filiaciones:
Gómez R.:
Department of Genetics, National Rehabilitation Institute, Mexico, D.F., Mexico
Cisneros B.:
Department of Genetics and Molecular Biology, Cinvestav-IPN, Mexico, D.F., Mexico
Casas L.:
Department of Genetics, National Rehabilitation Institute, Mexico, D.F., Mexico
Valdés-Flores M.:
Department of Genetics, National Rehabilitation Institute, Mexico, D.F., Mexico
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