CD13 is a novel mediator of monocytic/endothelial cell adhesion
Por:
Mina-Osorio P., Winnicka B., O'Conor C., Grant C.L., Vogel L.K., Rodriguez-Pinto D., Holmes K.V., Ortega E., Shapiro L.H.
Publicada:
1 ago 2008
Resumen:
During inflammation, cell surface adhesion molecules guide the adhesion and migration of circulating leukocytes across the endothelial cells lining the blood vessels to access the site of injury. The transmembrane molecule CD13 is expressed on monocytes and endothelial cells and has been shown to mediate homotypic cell adhesion, which may imply a role for CD13 in inflammatory monocyte trafficking. Here, we show that ligation and clustering of CD13 by mAb or viral ligands potently induce myeloid cell/endothelial adhesion in a signal transduction-dependent manner involving monocytic cytoskeletal rearrangement and filopodia formation. Treatment with soluble recombinant (r)CD13 blocks this CD13-dependent adhesion, and CD13 molecules from monocytic and endothelial cells are present in the same immunocomplex, suggesting a direct participation of CD13 in the adhesive interaction. This concept is strengthened by the fact that activated monocytic cells adhere to immobilized recombinant CD13. Fu
Filiaciones:
Mina-Osorio P.:
Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, United States
Winnicka B.:
Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, United States
O'Conor C.:
Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, United States
Grant C.L.:
Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, United States
Vogel L.K.:
Department of Medical Biochemistry and Genetics, Panum Institute, University of Copenhagen, Denmark
Rodriguez-Pinto D.:
Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, United States
Holmes K.V.:
Molecular Biology Program, University of Colorado at Denver, Health Sciences Center, Aurora, CO, United States
Ortega E.:
Univ Nacl Autonoma Mexico, Dept Immunol, Inst Invest Biomed, Mexico City 04510, DF, Mexico
Shapiro L.H.:
Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, United States
University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030-3501, United States
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