Antihypertensive and vasorelaxant activities of Laelia autumnalis are mainly through calcium channel blockade


Por: Vergara-Galicia J., Ortiz-Andrade R., Castillo-España P., Ibarra-Barajas M., Gallardo-Ortiz I., Villalobos-Molina R., Estrada-Soto S.

Publicada: 1 jul 2008
Resumen:
The aim of the present study was to evaluate the possible mechanism of the vasorelaxant action of methanol extract from Laelia autumnalis (MELa) in isolated rat aortic rings, and to establish its antihypertensive activity in vivo. MELa (0.15 -> 50 mu g/mL) induced relaxation in aortic rings pre-contracted with KCI (80 mM), showing an IC50 value of 34.61 +/- 1.41 mu g/mL and E-max value of 85.0 +/- 4.38% (in endothelium-intact rings) and an IC50 value of 45.11 +/- 4.17 mu g/mL and E-max value of 80.0 +/- 12.1% (in endothelium-denuded rings). Serotonin (5-HT, 1 X 10(-4) M) provoked sustained contraction, which was markedly inhibited by MELa (0.15 -> 50 mu g/mL) in a concentration-dependent and endothelium-independent manner. Pretreatment with MELa (15, 46,150, 300 and 1500 mu g/mL) also inhibited contractile responses to norepinephrine (NE 1 x 10(-11) M to 1 x 10(-5.5) M). In endothelium-denuded rings, the vasorelaxant effect of MELa was reduced partially by ODQ(1 mu M), but not by tetra

Filiaciones:
Vergara-Galicia J.:
 Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, C.P. 62209. Cuernavaca, Morelos, Mexico

Ortiz-Andrade R.:
 Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, C.P. 62209. Cuernavaca, Morelos, Mexico

Castillo-España P.:
 Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, C.P. 62209. Cuernavaca, Morelos, Mexico

Ibarra-Barajas M.:
 Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, DF, Mexico

Gallardo-Ortiz I.:
 Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, DF, Mexico

Villalobos-Molina R.:
 Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, DF, Mexico

Estrada-Soto S.:
 Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, DF, Mexico
ISSN: 15371891
Editorial
Elsevier Inc., 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA, Estados Unidos America
Tipo de documento: Article
Volumen: 49 Número: 1
Páginas: 26-31
WOS Id: 000258009300005
ID de PubMed: 18534917