Antihypertensive and vasorelaxant activities of Laelia autumnalis are mainly through calcium channel blockade
Por:
Vergara-Galicia J., Ortiz-Andrade R., Castillo-España P., Ibarra-Barajas M., Gallardo-Ortiz I., Villalobos-Molina R., Estrada-Soto S.
Publicada:
1 jul 2008
Resumen:
The aim of the present study was to evaluate the possible mechanism of the vasorelaxant action of methanol extract from Laelia autumnalis (MELa) in isolated rat aortic rings, and to establish its antihypertensive activity in vivo. MELa (0.15 -> 50 mu g/mL) induced relaxation in aortic rings pre-contracted with KCI (80 mM), showing an IC50 value of 34.61 +/- 1.41 mu g/mL and E-max value of 85.0 +/- 4.38% (in endothelium-intact rings) and an IC50 value of 45.11 +/- 4.17 mu g/mL and E-max value of 80.0 +/- 12.1% (in endothelium-denuded rings). Serotonin (5-HT, 1 X 10(-4) M) provoked sustained contraction, which was markedly inhibited by MELa (0.15 -> 50 mu g/mL) in a concentration-dependent and endothelium-independent manner. Pretreatment with MELa (15, 46,150, 300 and 1500 mu g/mL) also inhibited contractile responses to norepinephrine (NE 1 x 10(-11) M to 1 x 10(-5.5) M). In endothelium-denuded rings, the vasorelaxant effect of MELa was reduced partially by ODQ(1 mu M), but not by tetra
Filiaciones:
Vergara-Galicia J.:
Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, C.P. 62209. Cuernavaca, Morelos, Mexico
Ortiz-Andrade R.:
Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, C.P. 62209. Cuernavaca, Morelos, Mexico
Castillo-España P.:
Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001, Colonia Chamilpa, C.P. 62209. Cuernavaca, Morelos, Mexico
Ibarra-Barajas M.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, DF, Mexico
Gallardo-Ortiz I.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, DF, Mexico
Villalobos-Molina R.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, DF, Mexico
Estrada-Soto S.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Mexico City 54090, DF, Mexico
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