Src kinase regulates metalloproteinase-9 secretion induced by type IV collagen in MCF-7 human breast cancer cells
Por:
Cortes-Reynosa P., Robledo T., Macias-Silva M., Wu S.V., Salazar E.P.
Publicada:
1 abr 2008
Categoría:
Molecular Biology
Resumen:
Matrix metalloproteinases (MMPs) are a family of endopeptidases that collectively are capable to degrading all components of the extracellular matrix (ECM) and they have been implicated in several aspects of tumor progression, such as invasion through basement membrane (BM) and insterstitial matrices, angiogenesis and tumor cell growth. In particular, MMP-2 and MMP-9 have been associated with the ability of tumor cells to metastasize due to their capacity to degrade type IV collagen (Col-IV), the main component of BM, and to their elevated expression in malignant tumors. However, nothing is known about the regulation of MMP-9 secretion and expression in breast cancer cells stimulated with Col-IV Our results demonstrate that stimulation of MCF-7 cells with Col-IV promoted the secretion of MMP-9, as revealed by gelatin zymography and Western blotting using specific antibodies that recognized MMP-9. In addition, inhibition of Src and FAK kinase activity prevented MMP-9 secretion. In contr
Filiaciones:
Cortes-Reynosa P.:
Departamento de Biologia Celular. Cinvestav-IPN, Mexico, DF 07360, Mexico
Robledo T.:
Departamento de Biologia Celular. Cinvestav-IPN, Mexico, DF 07360, Mexico
Macias-Silva M.:
Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City 04510, DF, Mexico
Wu S.V.:
UCLA-CURE Digestive Diseases Research Center, UCLA, Los Angeles, CA 90073, United States
Salazar E.P.:
Departamento de Biologia Celular. Cinvestav-IPN, Mexico, DF 07360, Mexico
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