The Cys-Asn-Ser carboxyl-terminal end group is the pharmacophore of the amebic anti-inflammatory monocyte locomotion inhibitory factor (MLIF)
Por:
Morales-Martínez M.E., Silva-García R., Soriano-Correa C., Giménez-Scherer J.A., Rojas-Dotor S., Blanco-Favela F., Rico-Rosillo G.
Publicada:
1 mar 2008
Resumen:
The monocyte locomotion inhibitory factor (MLIF) is an anti-inflammatory oligopeptide produced by Entamoeba histolytica. Among its different effects, it inhibits locomotion of human monocytes, hence its original name. Previous experimental studies have shown that the anti-inflammatory properties of MLIF (Met-Gln-Cys-Asn-Ser) remained when aminoacid glutamine was substituted by a proline in the second position (pMLIF: Met-Pro-Cys-Asn-Ser). By changing the order of MLIF amino acids, the resulting scrambled oligopeptide (sMLIF: Gln-Cys-Met-Ser-Asn) has failed activity. By means of ab initio study at the Hartree-Fock and Density Functional Theory levels, it was found that MLIF and pMLIF peptides maintain a great structural similarity among the last three amino acids (...Cys-Asn-Ser) predicting a pharmacophore. The objective of this work was to experimentally verify in vivo and in vitro the existence of the pharmacophore group in MLIF. We assayed three tripeptides by respiratory burst and d
Filiaciones:
Morales-Martínez M.E.:
Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, México D.F. 06720, Mexico
Silva-García R.:
Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, México D.F. 06720, Mexico
Soriano-Correa C.:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Lab Quim Computac QFB, Mexico City 04510, DF, Mexico
Giménez-Scherer J.A.:
Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, México D.F. 06720, Mexico
Rojas-Dotor S.:
Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, México D.F. 06720, Mexico
Blanco-Favela F.:
Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, México D.F. 06720, Mexico
Rico-Rosillo G.:
Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, México D.F. 06720, Mexico
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