Inactivation of bacteria inoculated inside urinary stone-phantoms using intracorporeal lithotripters
Por:
Gutiérrez J., Álvarez U.M., Mues E., Fernández F., Gómez G., Loske A.M.
Publicada:
1 feb 2008
Resumen:
Intracorporeal lithotripsy is used to treat a high percentage of urinary calculi. Urinary calculi may contain bacteria, which might cause septicemia after lithotripsy; however, little is known about the effects of lithotripters on the viability of microorganisms inside renal calculi. The objectives of this study were to evaluate the bactericidal effect, and the potential effect on intra-bacterial protein release of four different intracorporeal lithotripters on Escherichia coli (E. coli) inoculated inside artificial kidney stones. An electrohydraulic, a pneumatic, an ultrasonic, and a holmium-laser lithotripter were used to pulverize a set of infected kidney stones inside a test tube containing a saline solution. Two different energy levels were tested per lithotripter. The stones were manufactured by mixing gypsum cement and Vel-mix-stone with a suspension containing E. coli. Results were analyzed by analysis of variance. The release of intracellular protein was measured with a spectr
Filiaciones:
Gutiérrez J.:
Nuevo Hospital Civil de Guadalajara, Universidad de Guadalajara, Guadalajara, Jalisco C.P. 44340, Mexico
Álvarez U.M.:
Univ Nacl Autonoma Mexico, Fac Quim, Posgrad Ciencias Quim, Mexico City 04510, DF, Mexico
Mues E.:
Nuevo Hospital Civil de Guadalajara, Universidad de Guadalajara, Guadalajara, Jalisco C.P. 44340, Mexico
Fernández F.:
Univ Nacl Autonoma Mexico, Ctr Fis Aplicada & Tecnol Avanzada, Dept Nanotecnol, Queretaro 76000, Mexico
Gómez G.:
Nuevo Hospital Civil de Guadalajara, Universidad de Guadalajara, Guadalajara, Jalisco C.P. 44340, Mexico
Loske A.M.:
Univ Nacl Autonoma Mexico, Ctr Fis Aplicada & Tecnol Avanzada, Dept Nanotecnol, Queretaro 76000, Mexico
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