Development and validation of liquid chromatography method for quantification of the active markers of Hintonia standleyana and Hintonia latiflora crude drugs


Por: Mata, R, Acevedo, L, Mendez-Bautista, DI, Guerrero-Analco, JA, Rivero, BE, Rodriguez, JM

Publicada: 1 ene 2008
Resumen:
The infusions prepared from the stem-bark of Hintonia latiflora (Sesse et Mocino ex DC) Bullock or Hintonia standleyana Bullock (Rubiaceae) are indistinctly used in Mexican traditional medicine for the treatment of non-insulin-dependent diabetes mellitus and malaria fevers. Suitable high-performance liquid chromatography (HPLC)-UV methods for quantitative determination of 5-O-[beta-D-apiofuranosyl-(1-->6)-beta-D-glucopyranosyl]-7-methoxy-3',4'-dihydroxy-4-phenylcoumarin (1) and 5-0-[beta-D-xylopyranosyl-(1-->6)-beta-D-glucopyranosyl]-7-methoxy-3',4'-dihydroxy-4-phenylcoumarin (2) in the infusions of H. standleyana and H. latiflora, respectively, were established. Coumarins I and 2 are the major antihyperglycemic compounds found in the infusions prepared from stem-bark of H. standleyana and H. latiflora, respectively, and are proposed as the active markers. The methods were found to be reliable, reproducible, and accurate.

Filiaciones:
Mata, R:
 Univ Nacl Autonoma Mexico, Fac Quim, Dept Farmacia, Mexico City 04510, DF, Mexico

Acevedo, L:
 Univ Nacl Autonoma Mexico, Fac Quim, Dept Farmacia, Mexico City 04510, DF, Mexico

Mendez-Bautista, DI:
 Univ Nacl Autonoma Mexico, Fac Quim, Dept Farmacia, Mexico City 04510, DF, Mexico

Guerrero-Analco, JA:
 Univ Nacl Autonoma Mexico, Fac Quim, Dept Farmacia, Mexico City 04510, DF, Mexico

Rivero, BE:
 Univ Nacl Autonoma Mexico, Fac Quim, Dept Farmacia, Mexico City 04510, DF, Mexico

Rodriguez, JM:
 Univ Nacl Autonoma Mexico, Fac Quim, Dept Farmacia, Mexico City 04510, DF, Mexico
ISSN: 13880209
Editorial
INFORMA HEALTHCARE, TELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLAND, Países Bajos
Tipo de documento: Article
Volumen: 46 Número: 1-2
Páginas: 105-110
WOS Id: 000252850700014