Constitutive STAT3 activation in peripheral CD3(+) cells from patients with primary Sjogren's syndrome
Por:
Ramos H.L., Valencia-Pacheco G., Alcocer-Varela J.
Publicada:
1 ene 2008
Resumen:
Objective: Signal transducers and activators of transcription (STATs) are crucial mediators of cytokine signalling. Constitutive activation of STATs, especially STAT3, has been reported in several diseases. Primary Sjgren's syndrome (pSS) is associated with overproduction of cytokines such as interleukin-10 (IL-10), although the mechanism by which this occurs is unknown. As STAT3 is a potent inducer of IL-10, this study focused on determining the pattern of STAT3 activation in peripheral lymphocytes from patients with pSS. Methods: Twelve pSS patients and 12 healthy age-matched control subjects were studied. Peripheral blood mononuclear cells (PBMCs) were isolated by gradient centrifugation. Phosphorylated STAT3 (pSTAT3) and also STAT3 expression were determined by flow cytometry in gated CD3(+) and CD19(+) lymphocytes. Similarly, pJak1 and pTyk2 were also determined in gated CD3(+) lymphocytes. Results: Although the protein expression of STAT3 was similar among controls and pSS patien
Filiaciones:
Ramos H.L.:
Department of Immunology and Rheumatology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City, Mexico
Molecular Immunology and Inflammation Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH, Bethesda, MD 20892-1820, United States
Valencia-Pacheco G.:
Department of Immunology, School of Medicine, Autonomous University of San Luis Potosí, Mexico
Alcocer-Varela J.:
Department of Immunology and Rheumatology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Mexico City, Mexico
National Institute of Medical Sciences and Nutrition Salvador Zubrián, Vasco de Quiroga 15, Tlalpan 14000, Mecico City, Mexico
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