Experimental Strategies to Manipulate the Cellular Levels of the Multifunctional Factor CTCF
Por:
González-Buendía E., Pérez-Molina R., Ayala-Ortega E., Guerrero G., Recillas-Targa F.
Publicada:
1 ene 2014
Resumen:
Cellular homeostasis is the result of an intricate and coordinated
combinatorial of biochemical and molecular processes. Among them is the
control of gene expression in the context of the chromatin structure
which is central for cell survival. Interdependent action of
transcription factors, cofactors, chromatin remodeling activities, and
three-dimensional organization of the genome are responsible to reach
exquisite levels of gene expression. Among such transcription factors
there is a subset of highly specialized nuclear factors with features
resembling master regulators with a large variety of functions. This is
turning to be the case of the multifunctional nuclear factor
CCCTC-binding protein (CTCF) which is involved in gene regulation,
chromatin organization, and three-dimensional conformation of the genome
inside the cell nucleus. Technically its study has turned to be
challenging, in particular its posttranscriptional interference by small
interference RNAs. Here we describe three main strategies to
downregulate the overall abundance of CTCF in culture cell lines.
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