Hyper, a hydrogen peroxide sensor, indicates the sensitivity of the Arabidopsis root elongation zone to aluminum treatment
Por:
Hernández-Barrera A., Velarde-Buendía A., Zepeda I., Sanchez F., Quinto C., Sánchez-Lopez R., Cheung A.Y., Wu H.-M., Cardenas L.
Publicada:
1 ene 2015
Resumen:
Emerging evidence indicates that some reactive oxygen species (ROS), such as the superoxide anion radical and hydrogen peroxide (H2O2), are central regulators of plant responses to biotic and abiotic stresses. Thus, the cellular levels of ROS are thought to be tightly regulated by an efficient and elaborate pro-and antioxidant system that modulates the production and scavenging of ROS. Until recently, studies of ROS in plant cells have been limited to biochemical assays and the use of fluorescent probes; however, the irreversible oxidation of these fluorescent probes makes it impossible to visualize dynamic changes in ROS levels. In this work, we describe the use of Hyper, a recently developed live cell probe for H2O2measurements in living cells, to monitor oxidative stress in Arabidopsis roots subjected to aluminum treatment. Hyper consists of a circularly permuted YFP (cpYFP) inserted into the regulatory domain of the Escherichia coli hydrogen peroxide-binding protein (OxyR), and is a H2O2-specific ratiometric, and therefore quantitative, probe that can be expressed in plant and animal cells. Now we demonstrate that H2O2levels drop sharply in the elongation zone of roots treated with aluminum. This response could contribute to root growth arrest and provides evidence that H2O2is involved in early Al sensing. © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license.
Filiaciones:
Hernández-Barrera A.:
Department of Plant Molecular Biology, Institute of Biotechnology, National Autonomous University of Mexico62210, Mexico
Velarde-Buendía A.:
Department of Plant Molecular Biology, Institute of Biotechnology, National Autonomous University of Mexico62210, Mexico
Zepeda I.:
Department of Plant Molecular Biology, Institute of Biotechnology, National Autonomous University of Mexico62210, Mexico
Sanchez F.:
Department of Plant Molecular Biology, Institute of Biotechnology, National Autonomous University of Mexico62210, Mexico
Quinto C.:
Department of Plant Molecular Biology, Institute of Biotechnology, National Autonomous University of Mexico62210, Mexico
Sánchez-Lopez R.:
Department of Plant Molecular Biology, Institute of Biotechnology, National Autonomous University of Mexico62210, Mexico
Cheung A.Y.:
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, United States
Wu H.-M.:
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, United States
Cardenas L.:
Department of Plant Molecular Biology, Institute of Biotechnology, National Autonomous University of Mexico62210, Mexico
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