Seroprevalence and major antigens recognized by sera from Trypanosoma cruzi-infected dogs from Jalisco, México
Por:
Martínez I., Martínez-Ibarra A., Arce-Fonseca M., Rodríguez-Morales O., Pérez-Morales D., Reyes López P.A., Espinoza B.
Publicada:
1 abr 2014
Resumen:
Chagas disease is a major endemic disease caused by the protozoan parasite Trypanosoma cruzi. This parasitic disease is widely distributed throughout Latin America, affecting 10 million people. There are also reports of canine infection in the southern part of the United States. Dogs are considered the predominant domestic reservoir for T. cruzi in many areas of endemicity. In México, dog infection by this parasite has been poorly studied. In this work 209 dogs from six villages in Jalisco, México, were assessed to detect anti-T. cruzi antibodies by ELISA and Western blot. Seventeen (17) seropositive dogs (8.1 %) were detected by both tests, representing a seropositive value similar to that found in some southern states of México where the infection is present. No statistical differences were observed concerning the age and sex of infected and non-infected dogs. The major antigens recognized by positive sera were 26, 32, 66 and 80 kDa. These proteins are candidates to develop a specifi c diagnostic method for canine Chagas. No antibodies against HSP16 protein were found in T. cruzi seropositive sera. This is the fi rst report of canine serology of Chagas disease in this central part of México. This report will contribute to the knowledge of the infection status of domestic reservoirs in the state of Jalisco, México. © 2013 Asociación Argentina de Microbiología. Published by Elsevier España, S.L. All rights reserved.
Filiaciones:
Martínez I.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Del. Coyoacán, México City, Mexico
Martínez-Ibarra A.:
Área de Entomología Médica, Centro Universitario del Sur, Universidad de Guadalajara, Ciudad Guzmán, Jalisco, Mexico
Arce-Fonseca M.:
Departamento de Biología Molecular, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Rodríguez-Morales O.:
Departamento de Biología Molecular, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Pérez-Morales D.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Del. Coyoacán, México City, Mexico
Reyes López P.A.:
Laboratorio de Inmunología Molecular y Proteómica, Dirección de Investigación, Instituto Nacional de Cardiología Ignacio Chávez, México City, Mexico
Espinoza B.:
Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Del. Coyoacán, México City, Mexico
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